CPI Supports GlycoSeLect to Develop High Throughput Glycan Analysis Platform for Biotherapeutics

CPI has contributed its expertise in biotherapeutics to support an Innovate UK funded project focused on developing and integrating novel recombinant prokaryotic lectins (RPLs) into a biosensor-based platform. The platform will enable simplified, rapid, high throughput and cost-effective characterisation and monitoring of the glycosylation of biotherapeutic products.

The collaboration between CPI, GlycoSeLect UK Ltd, ForteBio-Molecular Devices and Allergan Biologics Ltd addressed the need for new analytical approaches to satisfy the growing biotherapeutics market. As medicines, biotherapeutics have been shown to be highly effective in the treatment of a multitude of diseases. With more than two-thirds of biotherapeutics existing in a glycosylated form, it is important that the glycan profile is monitored throughout product development and manufacture.

Characterising the glycan profile of the biotherapeutic is a key product quality attribute, and is important in delivering a safe and efficacious medicine to the patient. However, the development and manufacture of these potentially life-changing treatments carries a high risk of failure, is technically challenging and is expensive.

CPI, operating from its Darlington-based National Biologics Manufacturing Centre, used GlycoSeLect’s glycan recognition technology and ForteBio-Molecular Devices’ biosensor-based analytical platform technology to create a novel glycan analysis platform with the capability to reduce costs and accelerate product development pipelines.

The project developed an assay platform to define the glycosylation profiles of biotherapeutics, using ForteBio-Molecular Devices’ Octet instrument to detect interactions between GlycoSeLect’s RPLs and a range of biotherapeutic proteins, which were provided by Allergan Biologics.

The assay platform, validated by the consortium partners, established linearity of response and limits of detection, robustness, reproducibility, and specificity. It was also found to be effective on both purified and in-process biotherapeutic proteins and allowed for real-time analysis.

The results demonstrated that combining GlycoSeLect’s innovative RPLs with Bio-Layer Interferometry (BLI) based assay technology fulfilled the main objective of the project; to create a rapid, cost-effective analytical platform. This platform is available for use with high throughput process development and in process control (IPC) approaches to enable glycan characterisation of a biotherapeutic product throughout development and manufacturing phases.

Dr John Liddell, Chief Technologist, Centre for Process Innovation, said: “CPI is delighted to have worked with GlycoSeLect UK Ltd as well as the other project partners, ForteBio-Molecular Devices and Allergan Biologics Ltd on this project supporting the development of an innovative, novel approach to rapidly analyse glycan profiles of biotherapeutic proteins. The rapid, high throughput platform developed for the key quality attribute of glycosylation will help to streamline development and manufacture of biotherapeutic proteins contributing to lower costs and faster development of new medicines.”

Robert Dunne, CEO of GlycoSeLect UK Ltd, said: “GlycoSeLect’s team were pleased to collaborate with their fellow researchers at CPI, ForteBio-Molecular Devices and Allergan to deliver a successful Innovate UK funded project. Our RPL technology has been shown to be the core of a high throughput analytical platform for glycan analysis of biotherapeutic products. On behalf of GlycoSeLect I want to thank our colleagues from CPI, Allergan ForteBio-Molecular Devices and InnovateUK for their input”

CPI Supports GlycoSeLect to Develop High Throughput Glycan Analysis Platform for Biotherapeutics

Follow NETPark On Social Media

Keep up to date with the latest NETPark news and events.

NETPark Logo

The North East Technology Park provides a dynamic and supportive environment to accelerate the growth of ambitious, innovative, high tech companies into global markets. NETPark encourages collaborative multidisciplinary links, driving innovation, enterprise and economic prosperity.
We provide companies with access to a focused and international community where talent flourishes, ideas are generated and businesses have the support and resources to compete with the best in the world.

NETPark North East Technology Park

NETPark, North East Technology Park,
Sedgefield, County Durham TS21 3FD

Call Us: (+44) 01740 625250
Email: enquiries@northeasttechnologypark.com
Web: northeasttechnologypark.com

The quality of the facilities at NETPark and the attitude of staff is first class. It has a really dynamic, innovative atmosphere.

Dr Neil Loxley, - Chief executive, IBEX

The proximity to the universities and the infrastructure are very important factors. Ultimately having a presence in Europe is very valuable to us and we intend to stay at NETPark for many years to come.

Dr Arnab Basu, - CEO, Kromek

When looking for premises which would support our growth, whilst we’d looked at other locations nothing came close to NETPark. The office is modern and well serviced and reflects the type of company we are.

Paul Ashley , - Co-Founder, PNTS

We’re delighted to have opened our new site at NETPark.  This will make it easier for us to attract the best engineering talent to the Tharsus team so that we can continue to deliver game-changing robotic systems for our customers.

Brian Palmer , - CEO, Tharsus

We initially came because of the printable electronics centre but access to a highly skilled semi conductor workforce in the North East has been a key factor in us staying and growing here, as well as the value for money office space and the access to funding.

Ken Williamson, - COO, PragmatIC Printing

CPI has developed a specialist offering to support SMEs looking to develop their printable electronics applications and our partnership with Business Durham has been instrumental in attracting several SMEs to locate here in the region.

Dr Jon Helliwell, - Director of Printable Electronics, CPI

It fits perfectly for us that we’re surrounded by like-minded companies at NETPark. There are a number of highly skilled people in the area who can help us grow our business. The facilities and services offered by NETPark are first class, they make office life simple and engaging.

Stuart Wilson, - CEO, Ascarii

Access to quality facilities was one of the main drivers for deciding to locate the business at the NETPark. We have been able to use this as a launch pad to link into support from CPI and world-class expertise from Durham University.

Steve Clements, - Managing Director, aXenic
2019-06-06T14:31:08+00:00